Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Novus Biologicals™ Rat Free Tri-Iodothyronine ELISA Kit (Colorimetric)
Assay has high sensitivity and specificity for detection of rat Free-T3
| Target | Free Tri-Iodothyronine |
|---|---|
| Specificity | For detection of rat Free-T3 |
| Kit Contents | Adhesive Strip (For 96 wells), Assay plate, Conjugate, HRP-avidin, Substrate B, Substrate A, Wash Buffer (20 x concentrate), Stop Solution, Standard Instruction manual |
| Product Type | ELISA Kit (Colorimetric) |
| Sample Type | Cell Lysates,Plasma,Serum,Tissue Homogenates |
| Sample Volume | 50 to 100 μL |
| Assay Sensitivity | 0.38pmol/L |
| Assay Range | 2pmol/L to 32pmol/L |
| Storage Requirements | Store the unopened product at 2° to 8°C. Protect from light. Do not use past expiration date. |
| For Use With (Application) | ELISA |
Invitrogen™ Human PSA (Free)/KLK3 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Aliases | PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | KLK3 |
| Total Assay Time | 4 hr 45 min |
| Assay Sensitivity | 45 pg/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 45-12000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P07288 |
| Regulatory Status | RUO |
| Target | PSA |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Gene Alias | Antigen; antigen, prostate specific; APS; Fletcher factor; gamma-seminoprotein; hK3; Kal3; Kal-3; Kallikrein 3; kallikrein 3, plasma; kallikrein B, plasma 1; kallikrein related peptidase 3; kallikrein-3; Kallikrein-3 (KLK3); kallikrein-related peptidase 3; Kalp1; KALP15; Kininogenin; KLK2A1; Klk3; KLK-3; Klkb1; P-30 antigen; Pk; Plasma kallikrein; Plasma kallikrein heavy chain; Plasma kallikrein light chain; Plasma prekallikrein; prostate specific antigen; prostate-specific (APS); prostate-specific antigen; PSA; Semenogelase; semininAntigen; antigen, prostate specific; APS; Fletcher factor; gamma-seminoprotein; hK3; Kal3; Kal-3; Kallikrein 3; kallikrein 3, plasma; kallikrein B, plasma 1; kallikrein related peptidase 3; kallikrein-3; Kallikrein-3 (KLK3); kallikrein-related peptidase 3; Kalp1; KALP15; Kininogenin; KLK2A1; Klk3; KLK-3; Klkb1; P-30 antigen; Pk; Plasma kallikrein; Plasma kallikrein heavy chain; Plasma kallikrein light chain; Plasma prekallikrein; prostate specific antigen; prostate-specific (APS); prostate-specific antigen; PSA; Semenogelase; seminin; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 354 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Rat IgG (Total) Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Uncoated ELISA Kit |
|---|---|
| Target Species | Rat |
| Conjugate | HRP |
| Total Assay Time | 24 hr 30 min |
| Assay Sensitivity | 1.6 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 1.6-100 ng/mL |
| Test Time | 1 hr 45 min |
| Regulatory Status | RUO |
| Target | Rat IgG |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| Gene Alias | Immunoglobulin G; ImmunoglobulinGImmunoglobulin G; ImmunoglobulinG; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Human TNF beta ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10.2% |
| Intraassay CV | 8% |
| Gene Symbol | LTA |
| Total Assay Time | 4 hr 10 min |
| Sample Volume | Plasma 100 μL, Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity | 4.6 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 15.6-1000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P01374 |
| Regulatory Status | RUO |
| Target | TNF beta |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4049 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Lymphotoxin-alpha, TNF superfamily member 1 |
Invitrogen™ Mouse Epiregulin (EREG) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Family | Growth Factors & Receptors |
|---|---|
| Target Species | Mouse |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 10 pg/mL |
| Research Discipline | Angiogenesis |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | Epiregulin |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 10 pg/mL to 6000 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | Ereg |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 10-6000 pg/mL |
| Accession Number | Q61521 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 13874 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | EPR |
| Storage Requirements | Storage of components varies. See protocol for specific instructions. |
|---|
Novus Biologicals™ Human Isocitrate Dehydrogenase 1/IDH1 ELISA Kit (Colorimetric)
Colorimetric ELISA Kit
| Assay Sensitivity | 9.38 pg/mL |
|---|---|
| Assay Range | 15.63 to 1000 pg/mL |
Invitrogen™ Human Cathepsin A/CTSA ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | CTSA |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.016 ng/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.016-10 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P10619 |
| Regulatory Status | RUO |
| Target | Cathepsin A |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 5476 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Novus Biologicals™ Human transgelin 2 - Ready-To-Use ELISA Kit (Colorimetric)
Colorimetric ELISA Kit
| Storage Requirements | Storage of components varies. See protocol for specific instructions. |
|---|
Thermo Scientific™ CaptureSelect™ tPA Ligand Leakage ELISA
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Thermo Scientific™ CaptureSelect™ tPA Ligand Leakage ELISA is designed to detect possible leached ligand of tissue plasminogen activator (tPA) purified using CaptureSelect tPA Affinity Matrix.
| Shipping Condition | Dry Ice |
|---|---|
| Product Type | Ligand Leakage ELISA |
| Product Line | CaptureSelect |
Invitrogen™ Mouse TIMP2 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | TIMP2 |
| Total Assay Time | 4 hr 45 min |
| Assay Sensitivity | 80 pg/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.0823-60 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P25785 |
| Regulatory Status | RUO |
| Target | TIMP2 |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Gene Alias | Collagenase inhibitor; CSC-21K; D11Bwg1104e; DDC8; etID32613.12; fa96h09; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2a; metalloproteinase inhibitor precursor; metalloproteinase inhibitor TIMP-2; metalloproteinase-2; similar to metalloproteinase inhibitor; testicular secretory protein Li 57; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP metallopeptidase inhibitor 2a; TIMP2; Timp-2; TIMP-2 protein; timp2a; tissue inhibitor of matrix metalloproteinase-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase 2 precursor; tissue inhibitor of metalloproteinase 2a; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinase-2 precursor; tissue inhibitor of metalloproteinases 2; tissue inhibitor of mettaloproteinase 2; wu:fa96h09; zgc:136726Collagenase inhibitor; CSC-21K; D11Bwg1104e; DDC8; etID32613.12; fa96h09; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2a; metalloproteinase inhibitor precursor; metalloproteinase inhibitor TIMP-2; metalloproteinase-2; similar to metalloproteinase inhibitor; testicular secretory protein Li 57; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP metallopeptidase inhibitor 2a; TIMP2; Timp-2; TIMP-2 protein; timp2a; tissue inhibitor of matrix metalloproteinase-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase 2 precursor; tissue inhibitor of metalloproteinase 2a; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinase-2 precursor; tissue inhibitor of metalloproteinases 2; tissue inhibitor of mettaloproteinase 2; wu:fa96h09; zgc:136726; D11Bwg1104e; Timp-2 |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 21858 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |